Overview of the PCRTiler oligonucleotide design process. The input sequences are first split into smaller regions according to the parameters defined by the user. A primer pair targeting each of these sub-regions will be designed. PCRTiler maximizes utilization of server resources by designing as many primer pairs in parallel as the server has processors. Candidate primer pairs are identified using Primer3, and BLAST is used to exclude primers with a high cross-hybridization potential (see text). A report is generated when all design tasks are finished.