Characterization of the kinetics of each T7-IVT amplification round. Two tissues were tested, i.e. germinal-stage oocytes and 8-cell embryos. ( A ) The aRNA production during the first round was followed using real-time PCR targeting the actin gamma 1 (ACTG1) and high-mobility group box 1 (HmgB1) transcripts. ( B ) The aRNA production during the second round was followed by measuring the incorporation of radiolabeled UTP.