Pathway analysis for (A) ribo-depletion RNA-seq, (B) poly-A-enrichment RNA-seq, (C) 1-color microarrays, and (D) 2-color microarrays. The top 15 pathways for the FRO groups (blue) are listed vertically. The level of enrichment of the corresponding FFPE groups (white-FFPE-18 h; grey-FFPE-3 weeks) is plotted. Pathway significance was calculated in IPA using a Fisher Exact test; −log(P-value) = 1.3 corresponds to a P = 0.05 (indicated in red). Venn diagrams indicate the overlap of each full list. For all analyses, only mapped DEGs with FDR P < 0.05 and fold change > ± 1.5 were used and only pathways with at least 4 DEGs were considered. Nrf2 Oxidative Stress Response was the only pathway that was enriched in all 12 groups (blue chevrons); p53 was enriched in 11/12 groups (grey chevrons). Full color version available online.