Figure 4.
Hcy and DHEA affect the histomorphology of the mouse ovary. (a) The histological staining (hematoxylin and eosin staining) of representative ovaries on day 22 of treatment. Survey views show rat ovaries of control, Hcy, DHEA, Hcy + DHEA. Scale bar =100 μm. Comparison of the thickness of the (b) theca cell layer and (c) granulosa cell layer, as well as the (d) number of corpora lutea in the mouse ovaries of four groups. (e) Comparison of the expression levels of AMH and AMH receptor 2 (AMHR2) of the follicles in the mouse ovaries of four groups (n = 8). *P < 0.05, one-way analysis of variance, with Tukey’s post hoc test. Ctl, control.