REGN3776 increases LPL activity and improves TG clearance in Angptl8^hum/hum mice. (a) Post-heparin plasma LPL and HL activity of chow-fed Angptl8^hum/hum mice treated with REGN3776 or control antibody (10 mg/kg, n=5/group). Post-heparin plasma was pooled and fractionated on a heparin column to separate HL and LPL, and TG hydrolase activity was measured. (b) Effect of REGN3776 treatment on plasma TG levels following a lipid tolerance test. Male Angptl8^hum/hum mice were treated with REGN3776 or control antibody (10 mg/kg, n = 5 per group) 4 days prior to the intravenous administration of Intralipid (2.5 µL/g of 20% Intralipid). Values are mean ± SEM. Statistical analysis was conducted by a Welch t test (a) and repeated measures two-way ANOVA with a Bonferroni’s posttest (b). **P < 0.01, ****P < 0.0001.