Peripherally administered F-ghrelin exclusively impacts the AP component of the DVC. (a and d) Representative images of DVC of vehicle- and F-ghrelin–treated mice (n = 4 and 5, respectively) subjected to chromogenic immunostaining against fluorescein and p-ERK, respectively. Insets in each image show high magnification of the areas marked in low-magnification images. Scale bars, 100 and 10 µm for the low- and high-magnification images, respectively. Bar graphs of quantitative analysis of fluorescein-IR (b and c) and p-ERK-IR ODs (e and f) are shown. Data represent the mean ± SEM. *P < 0.05 vs vehicle group. SC, subcutaneous.