Regulation of glucose metabolism and insulin action in Ldb1^+/− and control (CTL) littermates. (a, b) Islet LDB1 mRNA and protein levels following 14-week HF feeding. mRNA levels were normalized to Gapdh. Western blotting of nuclear extracts isolated from total pancreas. LDB1 band densitometry is included above each lane, relative to Actin loading control. (c) Fasting blood glucose on chow and following 10 weeks of HFD. Blood glucose excursion following an IP bolus of glucose (1.5 g/kg) on (d) chow diet and (e) after 8 weeks of HFD. Data represented as mean ± SEM. **P < 0.01; n = 10 to 14 mice/group.