Reovirus infection does not increase vascular permeability in vivo. A, Newborn (2–3 days) wild-type mice were inoculated perorally with either phosphate-buffered saline (PBS) or reovirus strain T1L at 5 × 106 PFU per mouse. At 4 and 8 days post-inoculation, mice were injected intraperitoneally with 0.1 mL 10% sodium fluorescein. B, Mice (7–8 days) were inoculated with 1 mg/kg lipopolysaccharide (LPS) and 3 hours later injected intraperitoneally with 0.1 mL 10% sodium fluorescein. Ten minutes later, all mice were euthanized and perfused. Brains were homogenized, and fluorescein content was determined using a microplate reader with 485 nm excitation and 528 nm emission filters. Fluorescein content in blood was used to normalize the fluorescein content in the brain. In the PBS- and LPS-treated groups, 2 to 3 mice were used; in the reovirus-infected group, 5 mice were used at both time points. *P < .05 by Student t test.
