Evaluation of the dose- and time-dependent inhibitory effect of PR66 given by the intraperitoneal route. (a) Increasing concentrations from 0.1 to 10 mg/kg per day of PR66 were administered from day −1 by the intraperitoneal route, whereas the mice in the cure 10 group were given 10 mg/kg per day of PR66 from day 2. Neurological disease scores (b) and mortality rates (c and d) were recorded daily. Mean score from each group was calculated and the numbers of mice in each group are shown in brackets. *P < 0.05 and ***P < 0.0001.