Effects of overexpressing MIR319a orthologs in Arabidopsis thaliana. (A) Sequence of miR319a in Brassica oleracea var. acephala. The noncanonical residue is highlighted. The miRNA is aligned to known miR319 targets, TCP4 and MYB65, with cleavage sites known from A. thaliana indicated by arrows (Palatnik et al. 2003, 2007). (B) Arabidopsis thaliana MIR319a, construct SD164. (C) Arabidopsis lyrata MIR319a, construct SD141. (D) Sibara virginica MIR319a, construct SD91. (E) Brassica oleracea MIR319a, seedling and rosette: construct SD165, flower: construct SD144. (F) Brassica oleracea MIR319a, mutated to the canonical miR319a sequence, seedling and rosette: construct SD166, flower: construct SD147. (G) Arabidopsis thaliana MIR319c, construct JP122 (Palatnik et al. 2007). (H) Brassica oleracea MIR319c, construct NW29. All seedlings and rosettes are of same age, respectively, and the pictures are to the same scale. See also supplementary table 5 (Supplementary Material online) for construct designations and details.