Mitochondrial respiration. A subgroup of preadipocytes O-BP (n = 11), O-GDM (n = 9), and O-T1DM (n = 10) were cultured in a seahorse plate until day 12 and oxygen consumption rate (OCR) was measured. (a) Basal respiration was first inhibited with oligomycin (O, inhibitor of oxidative phosphorylation), followed by treatment with FCCP (F, uncoupler of mitochondrial oxidative phosphorylation) and then antimycin A (A)/rotenone (R, inhibitor of mitochondrial respiratory chain). (b) Relative OCR given in percentage. Data are shown as mean ± SEM. *P < 0.05 (t test).