DHT increases RNF6 and AR contents and nuclear RNF6 localization in GCs from preantral and antral follicles in vitro. GCs isolated from preantral and antral follicles were cultured with DHT (0 to 1 µm, 24 hours), and changes in (a) RNF6 and (b) AR contents were assessed by Western blot. Results are expressed as means ± standard error of the mean (n = 4) and analyzed by two-way ANOVA and Bonferroni post hoc test. **P < 0.01 versus CTL. (c) Subcellular localization of RNF6 was analyzed by IF and is shown as percentage of total cells. Data were analyzed by two-way ANOVA and Bonferroni post hoc test. N+C RNF6 localization: **P < 0.01; ***P < 0.001 versus CTL. ^#P < 0.05; ^##P < 0.01 (DHT 1.0 µm versus DHT 0.1 µm). Normal rabbit IgG was used for negative control (N CTL). GAPDH, glyceraldehyde 3-phosphate dehydrogenase.