Renal Agt, hnRNP F/K, Nrf2, HO-1, and Keap1 expression in hyperinsulinemic-euglycemic mice. (a) Immunostaining of Agt, hnRNP F, and hnRNP K and (b) Nrf2, HO-1, and Keap1 (magnification ×200). (c) WB of Agt, hnRNP F, and hnRNP K and (d) Nrf2, HO-1, and Keap1 expression in isolated RPTs from WT mice after a 3-hour infusion with saline (Sal) or insulin (Ins) + d-glucose. (e) RT-qPCR of (i) Agt, (ii) hnRNP F, (iii) hnRNP K, (iv) Nrf2, (v) HO-1, and (vi) Keap1 mRNA expression in isolated RPTs from WT mice after a 3-hour infusion with saline (Sal; open bars) or Ins + d-glucose (solid black bars). Values are mean ± SEM, n = 8 per group. *P < 0.05; **P < 0.01. NS, not significant.