Isoenzyme analysis of elevated ALP. Mean (±SD) ALP activity for bone, gastrointestinal, and liver isoenzymes expressed as a percentage of the total ALP activity. Rats (3/sex/group) were administered 0.012 or 0.12 mg/kg of DAS181 for seven consecutive days by intravenous injection. Blood samples were taken at 24 h after the final dose and processed for ALP isoenzyme analysis.