Figure 11.
![Nuclear Localization Is Critical for NUDR Activity CV-1 cells were transfected with 2 μg of the reporter plasmid, pEnk77CAT6, and 1 μg of a CMV-promoter driven expression plasmid[ containing no insert (control) or the cDNAs for the following: human NUDR (hNUDR), hNUDR with a 14-amino acid deletion (hNUDR8), monkey NUDR (sNUDR), rat NUDR (rNUDR), and hNUDR with mutations (R302T, K304T, and R302T/K304T) in the NLS]. The results from this experiment are representative of two experiments and are expressed as the average fold induction over control (set at 1) ± sd of triplicate plates.](https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/mend/12/10/10.1210_mend.12.10.0181/2/mg1080181011.jpeg?Expires=1750533788&Signature=ZDzi96r0fYUmXPzDtq7F4H25oSE5WffEIewdw-2ESSWKce0O1PPzqgZes~wDyaYHElKv-sZpiQs9vEEOzF1JBEEvHIuYPB1G5nJQEd8iGaMSDALh1vDPzldunHXhfoRBsZ5noz1284BRSwvCSBzjNHBO7fOjmSUcXdP8Owu46SUrJW06MVAGvRqaniY99xqtIXoKLyXCeui-ADBVeKXXtTNv~hk-gxXo5igkeolghWY-4YR5Ex~yNUpDcw3xWLfCU-KijziZXbJH70Inp--DT4J4tIaxkhVY43lbFaXhBA69wuDT9wJzfzpWowe5osiwLY383J7-Wn2Niegqw0n5KA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Nuclear Localization Is Critical for NUDR Activity CV-1 cells were transfected with 2 μg of the reporter plasmid, pEnk77CAT6, and 1 μg of a CMV-promoter driven expression plasmid[ containing no insert (control) or the cDNAs for the following: human NUDR (hNUDR), hNUDR with a 14-amino acid deletion (hNUDR8), monkey NUDR (sNUDR), rat NUDR (rNUDR), and hNUDR with mutations (R302T, K304T, and R302T/K304T) in the NLS]. The results from this experiment are representative of two experiments and are expressed as the average fold induction over control (set at 1) ± sd of triplicate plates.
