Induction of PPARγ-CBP and PPARγ-SRC-1 Interaction by Known PPARγ Agonists A, TZDs induced interaction between hPPARγLBD and GST-CBP_1–453 in pull-down experiments. Purified hPPARγLBD (0.2 μg) was incubated with glutathione-bound GST-hCBP_1–453 (1–2 μg) in the absence or presence of 1μ m of each TZD compound (lane 1, input; lane 2, dimethylsulfoxide; lane 3, TZDB; lane 4, TZDC; lane 5, TZDD). Bound hPPARγLBD was eluted and analyzed by Western blotting. Using the HTRF approach, effects of TZDs on PPARγ-CBP interaction (B), PPARγ-SRC-1 interaction (C), and effects of PGs on PPARγ-CBP interaction (D) were analyzed in the presence of 10 nm SA/XL665, 10 nm biotin-CBP_1–453 or 10 nm biotin-SRC_568–780, 1 nm GST-PPARγLBD, and 2 nm α-GST-(Eu)K as described in Materials and Methods. For panels B and C, the symbols are: ○, TZDA; □, TZDB; ⋄, TZDC; •, TZDD; ▾, TZDE. The experiment was repeated three times with similar results.