Serum concentrations of Δ5-steroid sulfates across ages. LC-MS/MS was used to quantify serum concentrations of Δ5-steroid sulfates in the serum of males (n = 129, indicated by blue squares) and females (n = 118, indicated by pink circles) between ages 1.5 and 18 y. Age-centile curves were constructed from the raw data. Median is denoted by the black line and the 10th, 25th, 75th, and 90th percentiles are denoted by gray lines.