The number of hormone- or nonhormone-expressing endocrine cells in islets of WT and human HIP rats. Examples of CPHN cells in pancreas of 2- to 3-month (A) and 5- to 6-month (B) old HIP rat. Merged images of islet cells stained with insulin (white), endocrine cocktail (glucagon, somatostatin, pancreatic polypeptide, and ghrelin) (green), chromogranin A (red), and merged (yellow). Insets, higher magnification of endocrine cells where CPHN cells are indicated by arrows. (C) The number of insulin expressing beta cells was decreased in early onset diabetic (5–6 month old HIP rats) but not in nondiabetic (2- to 3-month-old HIP rats) compared to WT rats. (D) There was no change in the number of islet hormone expressing endocrine cells other than insulin in either 2–3 month or the 5–6 month in HIP rats compared to WT rats. (E) The number of CPHN cells is increased in both 2- to 3-month and 5- to 6-month-old HIP rats compared to WT rats. C, D, E: white bars: WT rats; black bars: HIP rats. All data were expressed as mean ± SEM, n = 4 per group, *P < .05.