Circulating miRNA regulation in T2DM patients.

A, Volcano plot of microarray analysis, on y-axis −Log₁₀P value (statistical difference in the variance between the sample groups), on x-axis the Log₂ of median expression changes between groups. The black dots represent unregulated miRNAs, blue dots represent up-regulated miRNAs, and red dots represent down-regulated miRNAs. B, The plot shows the regulated miRNA by alternative selection. Y-axis, the median signal intensity of the reference group samples; x-axis, the value of the test samples representative of the upper 75th percentile of the range of intensities subtracted from the 75th percentile value of the reference group. The blue dots represent up-regulated miRNAs, and red dots represent the down-regulated miRNAs. C, Histograms show the relative quantification of miR-22-3p gene expression ± SD in both groups after treatment (placebo group, gray; sildenafil group, black). ***, P < .001.