TR4 binds GR.

A and B, Murine (AtT20, A) or human pituitary corticotroph tumor cells (B) were lysed and subjected to immunoprecipitation using specific anti-TR4 or anti-GR antibodies, after which immunocomplexes were analyzed by immunoblot using anti-GR-, PR-, MCR-, and TR4-specific antibodies. C and D, AtT20 cells transfected with plasmids encoding TR4 and V5-tagged GR were harvested and immunoprecipitated with mouse IgG, anti-V5 (GR) antibody (C), or anti-TR4 antibody (D). The immunocomplexes were then analyzed by immunoblotting using specific antibodies to TR4 and V5 (GR). Data shown were representative of at least three independently repeated experiments.