Glis3 ubiquitous inactivation in adult mice leads to fulminant diabetes. (A) Eight-week-old Glis3^fl/fl/RosaCre^ERT2 male mice were treated with TAM or vehicle. Two weeks after treatment, gavage glucose tolerance test was performed after 6-hours fast. Plasma glucose was measured at time 0, 15, 30, 60, and 120 minutes after glucose injection (1.5 g/kg body weight) (N = 6). (B–F) Glis3^fl/fl/RosaCre^ERT2 mice were treated with TAM or vehicle, and 4 weeks after treatment, their body weights (B), blood glucose (C), plasma insulin (D), pancreatic insulin content (E), and Glis3 gene expression in the isolated islets were presented (N = 6). *P < 0.05; **P < 0.01; ***P < 0.001, versus vehicle-treated mice.