Examples of PDG cells expressing chromogranin A, NKx6.1, and endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, and ghrelin) in pancreas from an adult ND donor (A) and chromogranin A, NKx2.2, and endocrine cocktail in an adult T1D donor (B). Individual layers stained for NKx6.1/NKx2.2 (red), endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, and ghrelin) (white), and chromogranin A (green) are shown along with the merged (with 4′,6-diamidino-2-phenylindole) image. Insets, higher magnification of individual layers as marked by the yellow square in the low-power images. Yellow arrows indicate a PDG cell costaining for Nkx6.1, chromogranin A, and a cocktail of all the endocrine hormones (A) or Nkx2.2, chromogranin A, and a cocktail of all the endocrine hormones (B). Scale bars, 50 µm (for low-power images) and 25 µm (for insets).