Examples of PDG cells expressing Sox9-GATA4 in pancreas from an adult ND (A) and in an adult T1D donor (B). Individual layers stained for Sox9 (green) and GATA4 (red) are shown along with merged (with 4′,6-diamidino-2-phenylindole) images. Insets, higher magnification of individual layers as marked by the yellow square in the low-power images. Yellow arrows indicate a PDG cell costaining for Sox9 and GATA4. Scale bars, 50 µm (for low-power images) and 25 µm (for insets).