Expression of the complete IGF-IGFBP system in the control rainbow trout at the 3 developmental stages. A–F. Data for all characterized salmonid-specific IGFBP paralogues; each vertebrate IGFBP subtype has its own panel. A tree of IGFBP relationships is provided (adapted from Ref. 9) with different vertebrate IGFBP family members highlighted by different shading patterns and any teleost-specific paralogue pairs present being highlighted by different color font. Equivalent data are shown for genes coding the IGF-I and IGF-II hormones (G), the E3-ubiquitin ligase FBXO32 (H) and the fast muscle-specific structural protein TNNI2 (I). Different letters indicate significant differences between the 3 stages of development (n = 5) for each gene (letters are not valid for comparisons among different genes within the same panel). Accompanying details on statistics, including for other genes, is presented in Table 1.