Cyp27b1 ablation in ME modifies apoptosis, proliferation, cell cycle, and angiogenesis markers in mammary tumor tissue. A, IF staining for Bcl2 apoptosis regulator in (below) Western blotting. B, TUNEL staining for apoptosis and quantification histogram (n = 7 in each group; *, P = .045). C, IF staining of KI-67 proliferation marker. Below, Quantification. D, IF staining for cyclin D1 cell division. Below, Western blotting. E, IF staining for factor VIII angiogenesis marker. Below, Western blotting. F, IF staining for FLT1 angiogenesis marker. Below, Western blotting. All Western blottings illustrate a selection from 6 mice. For all, Cre⁻ are control and Cre⁺ are Cyp27b1-ablated animals. Scale bars, 50 μm (A and C–F) and 200 μm (B).