Relative Importance of Five NFAT Responsive Elements A, Plasmids with minimal thymidine kinase promoter (pTAL-Luc) containing the nucleotide sequence of each binding site (sites 1–5) were expressed without or with NFAT expression vector, and the ratio of the promoter activity [NFAT(+)/NFAT(−)] was determined. B, Basal promoter activity and the induction by NFAT were examined using wild-type (WT) or mutated GHRH gene 5′-promoter constructs in which one, multiple in combination, or all of the NFAT binding sites were destroyed. *, P < 0.05 vs. basal promoter activity.