Adaptive immune response induced in both tumors and tumor-draining lymph nodes. CT26-tumor bearing mice were treated with 11 mg/kg 1V270-micelles every fourth day and evaluated for gene expression by Nanostring in A or flow cytometry in B-I at indicated time points. Poor responders (PR) and escaped (esc) were defined based on tumor weights much higher than the group average and are shown in Fig. S11C–D with esc tumors being above 800 mg. (A) Heatmap of selected mRNA pathway scores from Nanostring. (B) Heatmap of viable cell composition in tumors. (C) Overall cell viability in tumors. (D) Immune infiltration in tumors. (E) AH-1 dextramer binding to CD8⁺ T cells in tumors. (F) CD8⁺ T cells to regulatory T cells (T_regs) ratio in tumors. (G) Heatmap of CD86⁺ phenotypes in tumor-draining lymph nodes (tdLNs). The number of macrophages (MΦ), monocytes and dendritic cells were limited in some samples but included as they were within the trend in the group. (H) Total number of immune cells per tdLN. (I) AH-1 dextramer binding to CD8⁺ T cells in tdLNs. Biological replicates were 4–5 in A, 2–12 in B–F, 2–12 in G – I, and 2–15 in H. Gating for B–F is shown in Figs S12 and S13. Gating for G-I is shown in Figs S14 and S15. Statistical analyses were Kruskal-Wallis with Dunn’s multiple comparisons tests (C–F and H and I). D, day; Mo-MDSCs, Monocytic Myeloid-Derived Suppressor Cells; PMos, Patrolling monocytes.