(A) mEos4b-STING and TBK1-HaloTag7 were stably expressed in STING/TBK1-double knockout MEFs. A typical overlapped image obtained by simultaneous observation of mEos4b-STING clusters (green, PALM image) and single molecules of TBK1-HaloTag7-SF650T (magenta) 60 min after stimulation with DMXAA. Arrowheads indicate the co-localization of STING clusters with single molecules of TBK1. (B) A graphical abstract illustrating cholesterol- and palmitoylation-dependent STING clustering at the TGN.