A comparison of the mean AUROC scores from the k-fold output of RWR_CV using the true comprehensive multiplex (blue) and 1m000 randomly rewired multiplex networks (red). (a) Illustration of a comparison of AUROC density across five folds using an individual set of genes curated for Jasmonate signaling obtained from MAPMAN. The true comprehensive multiplex (blue) has a mean AUROC across five folds of 0.993, whereas the 1,000 rewired multiplexes have an average mean AUROC across five folds of 0.498. (b) Depiction of a comparison of AUROC density across five folds using an individual set of genes curated for heat stress signaling obtained from MAPMAN. The true comprehensive multiplex has a mean AUROC across five folds of 0.766. The average mean AUROC across five folds for the 1,000 rewired multiplexes is 0.467. (c) Illustration of a comparison of the average AUROC density across 25 gold standard gene sets generated from shared MAPMAN terms including Jasmonate and heat stress signaling. The true comprehensive multiplex has an overall average mean AUROC of 0.91 across all 25 gold standard gene sets, whereas the 1,000 rewired multiplex networks have an overall average AUROC of 0.489 across all 25 gold standard gene sets, illustrating that the true comprehensive multiplex has meaningful biological connections compared to the completely random connections found across the 1,000 rewired multiplex networks.