Eosinophil depletion reduced PNE-specific antibodies and blocked anaphylaxis in iPhil^+/− FT^+/− neonatal mice. iPhil^+/− FT^+/− pups and FT^+/− pups were treated as described in Fig. 2A. Sera from iPhil^+/- FT^+/− neonatal mice at 4 h after the last sensitization (PND18) were measured by ELISAs for PNE-specific IgE (A), IgG1 (B), and IgG2b (C). (D, E) The pup change in body temperatures were monitored at PND19 at the indicated timepoints upon oral PNE challenge. (A–C) *P < 0.05 compared to all other groups. +P < 0.05 compared to all other DTX-treated groups. (D, E) *P < 0.05 for area under curve as compared to all groups without asterisks. Alt, Alternaria Alternata extract; DTX, diphtheria toxin; FT^+/−, flaky tail mice heterozygous for filaggrin and mattrin mutations; iPhil^+/−, mice heterozygous for inducible eosinophil deficiency; PNE, peanut extract.