(A) Reduced mRNA levels of SLC25A11 in tumor samples from the patient with Carney-Stratakis syndrome (CSS). Bars represent the mean ± SD from replicates. CSS sample (patient sample): Refers to the RNA obtained from the paraganglioma (PGL) of the CSS patient. Other samples: Includes pheochromocytoma (PHEO) and PGL from patients harboring pathogenic variants in Cluster 1 (SDHB, n = 2; SDHD, n = 1; VHL, n = 1) and Cluster 2 (RET, n = 5; TMEM127, n = 2). The ACTB gene was used as an endogenous control. (B) Western blot analysis of SLC25A11 protein levels in PHEO and PGL samples from the CSS patient. The gene variant for each sample is indicated in the figure. β-Actin was used as a loading control, and protein loading was further verified by Ponceau S staining.