Transcriptome and proteome analysis of gene products involved in CAM-metabolism. A) The tpm values for CAM transcripts in K. laxiflora at mid-day = Zeitgeber time 8 (zt8) and mid-night = zt20, naming based on Arabidopsis homologues. Scale is logarithmic. Highlighted names indicate significantly differentially expressed transcripts (q-value < 0.01). B) LFQs for CAM proteins in K. laxiflora at mid-night and mid-day. Note that y-axis is logarithmic and starts at 105. Names based on Arabidopsis homologues. Highlighted names indicate differentially abundant proteins (q-value <0.05 or only detected at 1 time point) and gray names indicate proteins that were not identified at both time points. Gene IDs are sorted based on their protein's involvement in the different groups I: carboxylation by PEP carboxylase; II: vacuolar storage; III: malate release and decarboxylation; IV: carbon storage and breakdown; V: gluconeogenesis. Abbreviations: ADG, ADP glucose pyrophosphorylase; ALMT, aluminum-activated malate transporter; AMY, alpha amylase; APL, glucose-1-phosphate adenylyltransferase; BASS, bile-acid sodium symporter; CA, carbonic anhydrase; DiT, dicarboxylate translocator; DPE, disproportionating enzyme; ENO, enolase; GBSS, granule-bound starch synthase; GPT, G6P phosphate transporter; HXK, hexokinase; MDH, malate dehydrogenase; NAD(P)-ME, NAD(P) dependent malic enzyme; PEP-CK, phosphoenolpyruvate carboxykinase; PGM, phosphoglucomutase; PHS, starch phosphorylase; PPase, pyrophosphatase; PPC, PEP carboxylase; PPCK, PPC kinase; PPDK, pyruvate, orthophosphate dikinase; PPT, PEP/phosphate translocator; RP, regulatory protein; SBE, starch branching enzyme; SS, starch synthase; TDT, tonoplast dicarboxylate transporter; TPT, triosephosphate/phosphate translocator.
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