GAS5 binds TPM4 in cytoplasm of myometrium cells. (A) Distribution of GAS5 in myometrium cells. Approximately 90% of GAS5 was distributed in the cytoplasm. (B) RNA pull-down and silver staining were done to find GAS5 related binding proteins. (C) Western blotting was done to find TPM4 in the pulldown protein lysis. (D) RIP was conducted to find GAS5 integrating TPM4. (E) Immunofluorescence staining for TPM4 (green) and FISH staining for GAS5 (red) and DAPI staining for nucleus (blue) were conducted in the same myometrium cell fixed slide, indicating the binding of GAS5 and TPM4 in cytoplasm of myometrium cells. ^****P < .0001.