GAS5 promotes contractions in primary myometrium cells. (A) Human primary myometrium cells were extracted and stained by α-SMA antibody. 100% of cells were α-SMA positive. (B) Transfection efficiency in primary myometrium cells with specific GAS5 siRNAs and GAS5 overexpression plasmid. (C) 3D collagen gel matrix was tested in myometrium cells after GAS5 siRNAs and GAS5 plasmid transfected. (D) Progesterone receptors (PRs) and contraction-associated proteins (CAPs) mRNA were detected by quantitative real-time PCR (qRT-PCR). (E) PRs and CAPs proteins were analyzed by western blotting. The gray values were quantified with the analysis software. *P < .05. ^#P < .05.