Co-expression modules generated by the SciGeneX algorithm on STs of a human thymus section. (A) H&E image of human thymus and matching spots annotated into eight topological areas. Medullary areas appear as internal zones with less intense labeling. (B) Spot clusters as identified by the FindClusters Seurat method (default parameters). The regions surrounded by a thin black line corresponds to the Seurat spot cluster associated with the medulla (Seurat spot cluster #4 in legend). (C) Scaled intensity per spot for 12 co-expression modules generated by the SciGeneX algorithm. To ease comparison, the Seurat spot cluster #4 is also depicted with a thin white line in all panels from (C).