Exogenous METTL7A improves the developmental potential of bovine IVP embryos. (A) The cleavage OF METTL7A^OE embryos (n = 29) compared to the vehicle control (n = 18). (B) The blastocyst rate of METTL7A^OE embryos (n = 11) compared to the control (n = 11). (C) A representative image of bovine METTL7A^OE embryos, scale bar = 100 μm. (D) Immunostaining analysis of CDX2 (TE marker) and SOX2 (ICM marker) in METTL7A^OE embryos compared to the control, scale bar = 50 μm. (E) The total TE cell number counts between METTL7A^OE blastocysts (n = 5) compared to the control (n = 5). (F) The total ICM cell number counts between METTL7A^OE blastocysts (n = 5) compared to the control (n = 5). (G) The TE/ICM cell number ratio between METTL7A^OE blastocysts compared to the control. (H) A representative bright field image of day 12 (E12) elongated embryos from METTL7A^OE and control embryo transfer, scale bar = 500 μm. I. The serum INF-tau levels of surrogate cows with METTL7A^OE embryo transfer (n = 2) compared to the control (n = 2) on the day of flushing. J. Immunostaining analysis of CDX2 (TE marker), SOX2 (embryonic disc marker), and GATA6 (hypoblast marker) in E12 embryos flushed out from the METTL7A^OE and control embryo transfer, scale bar = 50 μm.