Knockdown of CXCL12 and CD55 decreases the proliferation and migration of murine fibroblasts. Knockdown of CXCL12, CD55, and CD90 in murine fibroblasts 3T3-J2 was stably established. Representative image from the colony formation assay, showing strongly decreased proliferation after KD of A, CXCL12, C, CD55, and no difference after KD of E, CD90. B, D, and F, Bar graph represented the statistical results of the relative colony area (n = 4 technical replicates). Representative phase-contrast microscope images showing the area covered by the cells at 0 and 24 hours after wounding, showing decreased wound healing after KD of G, CXCL12, I, CD55, and no difference after KD of K, CD90. H, J, and L, Quantification of the wound healing assay. The data were normalized to the wound width of each group at 0 hours (n = 4–5 technical replicates). M, Quantitative polymerase chain reaction (qPCR) analysis of 3T3 cells after KD of CXCL12. Bar graph of relative expression of Il-6, Tgf-β, Il-11, α-sma, Cxcl5, Cxcl1, and Ccl2. N, 3T3 cells were incubated with 50 ng/mL recombinant CXCL12 protein for 6 hours and qPCR analysis was performed. Bar graph of relative expression of the same genes. All experiments were repeated 3-4 times independently. Nonpaired two-tailed t-test was performed. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, and ****P ≤ 0.0001.