Resistant mice generate more immune memory cells during primary P. yoelii infection. C57BL/6 mice from Taconic Biosciences (Resistant) and Charles River Laboratories (Susceptible) were infected with P. yoelii 17XNL–infected red blood cells. All data in Fig. 1 are representative of 2 independent experiments. (A) Percentage of red blood cells infected with P. yoelii on indicated days p.i. (n = 4) (means ± SEM). Area under the parasitemia curve (AUC) was analyzed by Wilcoxon matched-pairs signed-rank test. (B) Experimental timeline schematic. Spleens were harvested on d 0, 7, 14, 28 or 31, and 56 or 67 p.i. with P. yoelii. Created with BioRender.com. (C and E) Representative FACS plots for GC-derived MBCs (CD19+CD38+GL7-CD73+CD80+), Th1-like CD4 memory T cells (CD3+CD4+Ly6c+CD44+), and Tfh-like CD memory T cells (CD3+CD4+Ly6c-CD44+) at d 14 p.i.. (D and F) Total number of GC-derived MBCs, Th1-like CD4 memory T cells, and Tfh-like CD memory T cells present in the spleen at indicated time points (n = 4) (means ± SEM) analyzed using Mann–Whitney test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.