Figure 5
Protein tertiary structure-based analyses. (A) Distributions of protein richness per cell. (B) Relative proportion of total reads that either did not map to the assembled transcriptome, were assigned as ribosomal, or mapped to unannotated contigs or structurally annotated proteins (C) Venn diagram showing the number of shared and exclusive protein sets among postclustering groups. (D) Venn diagram showing the number of shared and exclusive protein sets that were differentially expressed among the postclustering groups. (E) Distribution of the predicted protein structures on protein sequence length (no. of amino acids: small (0, 200], medium (200, 400], large (400, 800]), and structure confidence (pLDDT scores: good (0.5, 0.7], high (0.7, 0.9], very high (0.9, 1]) axes. (F) Summary of KEGG pathways associated to multiple differentially expressed genes (n = no. of proteins in pathway, m = no. of those proteins recovered). Functions and pathways also identified by sequence homology (Fig. 3) include transcription and translation (ribosome, protein processing in endoplasmic reticulum, and proteasome), photosynthesis (porphyrin metabolism, photosynthesis, and carbon fixation in photosynthetic organisms), and cell metabolism (glycolysis, pyruvate metabolism, citrate cycle, fatty acid biosynthesis, and elongation) associated pathway groups.

Protein tertiary structure-based analyses. (A) Distributions of protein richness per cell. (B) Relative proportion of total reads that either did not map to the assembled transcriptome, were assigned as ribosomal, or mapped to unannotated contigs or structurally annotated proteins (C) Venn diagram showing the number of shared and exclusive protein sets among postclustering groups. (D) Venn diagram showing the number of shared and exclusive protein sets that were differentially expressed among the postclustering groups. (E) Distribution of the predicted protein structures on protein sequence length (no. of amino acids: small (0, 200], medium (200, 400], large (400, 800]), and structure confidence (pLDDT scores: good (0.5, 0.7], high (0.7, 0.9], very high (0.9, 1]) axes. (F) Summary of KEGG pathways associated to multiple differentially expressed genes (n = no. of proteins in pathway, m = no. of those proteins recovered). Functions and pathways also identified by sequence homology (Fig. 3) include transcription and translation (ribosome, protein processing in endoplasmic reticulum, and proteasome), photosynthesis (porphyrin metabolism, photosynthesis, and carbon fixation in photosynthetic organisms), and cell metabolism (glycolysis, pyruvate metabolism, citrate cycle, fatty acid biosynthesis, and elongation) associated pathway groups.

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