Differential bacterial elimination by Mtb H37Rv infected neutrophils from HR and LR TB patients. Neutrophils from TB patients were infected with Mtb H37Rv (MOI: 5) for 24 h. (A) CFU were determined by culturing cell lysate and enumerating the bacteria. Each bar represents the media (CFU x 10⁴/500,000 neutrophils) ± SEM. Black and grey bars corresponding to HR or LR patients respectively. Statistical differences were calculated using the paired t-test (*P < 0.05) or unpaired t-test (^#P < 0.05). N, HR = 3; N, LR = 3. (B) Necrosis and apoptosis were evaluated by flow cytometry using Annexin-V and a viability cell stain. The graph shows the percentages of viable, necrotic and apoptotic cells ± SEM. Viable cells: Annexin-V⁻/viability stain⁻; Apoptotic cells: Annexin-V⁺/viability stain^-; Necrotic cells: Annexin-V⁺/viability⁺.