Levels of activation markers on neutrophils from high (HR) and low (LR) responder TB patients. Neutrophils from HR and LR TB patients were incubated at 2.5 × 10⁶ cells/mL during 2 h in the presence or absence of Mtb-Ag (10 μg/ml). Then, SLAMF1, CD69, CD11b and PD-L1 expression was determined by flow cytometry. Bars represents the mean values of the percentage of (A) SLAMF1⁺, (B) CD69⁺, (D) PD-L1⁺ or the mean of the MFI ± SEM of (C) CD11b neutrophils. MFI was calculated for at least 12,000 events/cell culture condition. Statistical differences were calculated using the Wilcoxon signed rank test for paired samples (*P < 0.05; **P < 0.01; ***P < 0.001) or the Mann-Whitney nonparametric test for unpaired samples (^#P < 0.05; ^####P < 0.0001). N, HR = (A: 10; B: 8; C: 7, D: 8); N, LR = (A: 11; B: 14; C: 6, D: 15).