Expression of pro- and anti-inflammatory markers in neutrophils from high (HR) and low (LR) responder TB patients. Neutrophils were isolated from HR and LR TB patients. Then, ICAM-1, Fas and CXCR2 surface expression was evaluated by flow cytometry before (A–C) and after 2 h of Mtb-Ag stimulation (10 μg/ml) (D–F). Bars represent the mean of the median fluorescence intensity (MFI) ± SEM. MFI was calculated for at least 12,000 events/cell culture condition. Statistical differences were calculated using the Wilcoxon signed rank test for paired samples (D–F, Mtb-Ag vs medium; *P < 0.05; **P < 0.01; ***P < 0.001) or the Mann-Whitney nonparametric test for unpaired samples (A–F, HR vs LR patients; ^#P < 0.05; ^##P < 0.01). Number (N), HR=(A: 6; B: 7; C: 6, D: 6; E: 7; F: 6); N, LR=(A: 11; B: 8; C: 9, D: 13; E: 14; F: 12).