Effect of icariin on the fibrosis of penile cavernous tissue in DM rats. (A) Representative images (15×) of Masson's trichrome staining showing smooth muscle cells (SMCs) and collagen fibers. (B) Semiquantitative analysis of the SM/C ratio of each group. (C) NO levels in each group were measured using ELISA. (D) Western blot analysis of the expression of eNOS and peNOS in penile cavernous tissue from each group. (E, F) Semiquantitative analysis of eNOS and p-eNOS levels and the p-eNOS/eNOS ratio in penile cavernous tissue from each group. And P < .05 vs the control group. ^*P < .05 vs the control + ICA group. ^#P < .05 vs the DM group.