Effect of icariin on pyroptosis in the penile cavernous tissue of DM rats. (A) Representative images (20×) of the expression of caspase-1 in the penile cavernous tissue of the rats in each group, as determined by an immunofluorescence assay. The blue fluorescence represents DAPI. Green fluorescence represents CD31 (L) or α-SMA (R). Red fluorescence represents caspase-1. (B) Semiquantitative analysis of the percentage of caspase-1-positive penile cavernous tissue in each group. (C) Western blot analysis of the expression of caspase-1 and GSDMD in the penile cavernous tissue of each group. (D, E) Semiquantitative analysis of caspase-1 and GSDMD in penile cavernous tissue from each group. & P < .05 vs the control group. ^*P < .05 vs the control + ICA group. ^#P < .05 vs the DM group.