Figure 3.
Assessment of the cloned amplicon. (a) Blue/white colony counts for controls and vector ± insert (amplicon). (b) Gel image from a D5000 TapeStation trace to visualize the restriction digest evaluation of minipreps from 20 white colonies. (c) IGV visualization of Sanger sequencing results.

Assessment of the cloned amplicon. (a) Blue/white colony counts for controls and vector ± insert (amplicon). (b) Gel image from a D5000 TapeStation trace to visualize the restriction digest evaluation of minipreps from 20 white colonies. (c) IGV visualization of Sanger sequencing results.

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