Fig. 1.
Composite figure with four panels demonstrating photoperiod effects on juvenile hormone synthesis and related gene expression in Chrysoperla nipponensis. (A) Bar chart shows JH titers exhibited no significant difference between LD and SD females at 1, 3, and 5 d of C. nipponensis after emergence. JH titers of LD females increased to 300-fold and significantly higher than that of SD females at 10 d of C. nipponensis after emergence, while there was no significant difference between LD and SD females at 1, 3, and 5 d. Bar charts reveal: Jhamt expression (panel B), Kr-h1 expression (panel C) and Vg expression (panel D) were lower in SD females. Data presented as mean ± SE with asterisks indicating intergroup significance (*p<0.05, **p<0.01). Axes: horizontal scale represents experimental timeline (1, 3, 5, 10 days), vertical scales show relative expression levels (panels B-D) and JH III titter (panel A). LD: long-day (15:9 h light:dark); SD: short-day (9:15 h light: dark)

Effects of photoperiod on JH synthesis and JH-related gene expression in C. nipponensis. (A) The JH III (ng/g female lacewings) in C. nipponensis reared under LD and SD photoperiod. Expression profiles of Jhamt (B), Kr-h1 (C), and Vg (D) in intact C. nipponensis reared under LD and SD photoperiod. Data are shown as mean ± SE. Student’s t-test was used to analyze the statistical significance of the difference between the means of the two treatment groups (*P < 0.05, **P < 0.01). *indicates that the gene expression of C. nipponensis is significantly different on the same day under the long and short photoperiods (*P < 0.05; **P < 0.01), LD: long-day (15:9 h light:dark); SD: short-day (9:15 h light: dark).

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