Gavage of C. sporogenes and IPA treatment inhibit platelet aggregation and thrombus formation in WT mice. (a and b) Platelet aggregation and FeCl₃-injured thrombosis in mesenteric arteriole of mice treated with C. sporogenes or vehicle by gavage. WT mice were randomly administered with C. sporogenes or vehicle (100 μL LB containing 20% glycerol per mouse) for 10 consecutive days and then used for platelet aggregation induced by ADP (10 μmol/L) or collagen (0.5 μg/mL) in PRP and FeCl₃-injured thrombosis experiments. Representative aggregation tracings and summary data are presented in (a) (n = 5, biologically independent animals per group). Typical thrombus formation and the final and first occlusion time are presented in (b) (n = 10, biologically independent animals per group). (c and d) Platelet aggregation and FeCl₃-injured thrombosis in mesenteric arteriole of mice gavaged with IPA or vehicle. WT mice were randomly treated with IPA (20 mg/kg/day) or vehicle (sterile PBS, 100 μL/day) by gavage for 10 consecutive days, and then used for platelet aggregation in PRP (c) (n = 5, biologically independent animals per group) and FeCl₃-injured thrombosis in mesenteric arteriole (d) (n = 10, biologically independent animals per group). Data are shown as mean ± SEM. ^**P < 0.01. Data were analyzed using unpaired Student’s t-test (a, b, c, and d).