IF attenuates platelet activation and thrombosis in vivo. (a and b) Schematic diagram of AL or IF treatment for CAD patients (a) and representative platelet aggregation tracings of PRP induced by 10 μmol/L ADP (b). CAD patients underwent 10 consecutive days of AL or IF diet and their blood was drawn for platelet preparation and aggregation (n = 10, biologically independent individuals per group). (c) Schematic diagram of experiments presented in (d), (e), and (f). ApoE^−/− mice were randomly treated with AL or IF diet for 10 days and then used for platelet aggregation (d), FeCl₃-injured thrombus formation in mesenteric arteriole (e) experiments, or MCAO models (f). (d) Representative tracings and summary results of platelet aggregation in AL and IF-diet ApoE^−/− mouse induced by 10 μmol/L ADP or 0.5 μg/mL collagen, using PRP (n = 10, biologically independent animals per group). (e) Representative images of FeCl₃-injured thrombus formation in AL and IF-diet ApoE^−/− mice at 0, 6, and 12 min, respectively. Summary results of the first and final occlusion time are presented below (n = 10, biologically independent animals per group). (f) Representative pictures of the MCAO model conducted on AL and IF-diet ApoE^−/− mice. Summary results of infarction ratio, Bederson score, and Grip test performed 24 h after MCAO are shown (n = 10, biologically independent animals per group). Data are shown as mean ± SEM. ^**P < 0.01. Data were analyzed using unpaired Student’s t-test (b, d, e, and infarction ratio in f) and U test (Bederson score and Grip test in f).