Gene set enrichment analysis (GSEA) of gene expression differences between treatments in cumulus cells. (A) Volcano plot of 283 DEGs modulated by pro-cumulin in CC-MII-CAPAIVM pro-cumulin. (B, C) Charts show activated and inhibited pathways from Hallmark library and gene ontology (GO) processes, respectively. (D–I) Heatmaps showing clustering of genes involved in the activation or inhibition of biological pathways. (J) Venn diagram displaying the number of DEGs detected in CC-MII-CAPAIVM pro-cumulin compared to in CC-MII-CAPA pro-cumulin. A total of 13 DEGs from CC-MII-CAPAIVM pro-cumulin are still present in in CC-MII-CAPA pro-cumulin. Volcano plot of 49 DEGs are modulated by pro-cumulin in CC-MII-CAPA pro-cumulin. (K) Venn Diagram displaying the total number of transcripts detected in CCs from GV oocytes compared to CCs from MII oocytes. A total of 1852 transcripts are specific to CCs-GV, while 1127 transcripts are specific to CCs-MII. MII, metaphase II; DEGs, differentially expressed genes; CC, cumulus cells; GV, germinal vesicle; CAPA, capacitation.