Gene set enrichment analysis (GSEA) of gene expression differences between treatments in GV oocytes. (A) Volcano plot of 248 differentially expressed genes identified as being modulated by pro-cumulin in GV oocytes. (B) The top 20 gene ontology pathways that are significantly (FDR <0.1) enriched for downregulated genes. (C–G) Heatmaps showing supervised clustering of genes involved in the inhibition or activation of gene ontology pathways. GV, germinal vesicle; FDR, false discovery rate.